| Sterol and triterpenoid glycoside TESTS | 1 Antimony trichloride test: PINK
2 Tetranitro methane test: YELLOW |
| Specific tests for sterol | 1 Liebermann Burchard test: VIOLET RING
2 Salkowski test: YELLOW COLORED RING TURN TO RED |
| Specific tests: For c- types of anthraquinone
glycosides | 1 Modified brontrager’s test: PINK TO RED COLOR |
| Specific tests for cyanogenetic glycoside: | 1 Cuprocyanate test: Generation of HCN gas with the appearance of stain
2 Ferriferrocyanide test: PRUSSIAN BLUE COLOR
3 Precipitation of mercury from HgNO3:
Generation of HCN gas by → reduction of aqueous mercurous nitrite solution → metallic Hg |
| Saponin glycosides TESTS | 1 Haemolysis test: appearance of ruptured red blood cells
2 Foam test: GENERATION OF FROTHS |
| Flavonoid glycosides TESTS | 1 Shinoda test:
RED COLOR: PRESENCE OF FLAVONOIDS
DEEP RED COLOR TURN TO MAGENTA: PRESENCE OF dihydro flavonoids
2 Ammonia test: APPEARANCE OF YELLOW SPOT
3 Vanilin HCl test: PINK COLOR |
| Coumarin glycoside TESTS | 1 Fluorescence test: Generation of blue – green fluorescence
2 Ferric chloride test: → appearance of dark green color → turned to yellow after some time on addition of conc. HNO3 |
| Cardiac Glycosides TEST | SPECIFIC TEST
1 3,5-dinitro benzoic acid test: → PINK COLOR
GENERAL TEST
1 Legal’s test: PINK COLOR
2 Baljet test: yellow to orange colour
3 Keller-Killiani test: REDDISH BROWN LAYER TURNS TO BLUISH GREEN
4 Raymond's test: VIOLET COLOR |
