level: Level 1 of Genetic Engineering
Questions and Answers List
level questions: Level 1 of Genetic Engineering
| Question | Answer |
|---|---|
| How do bacteria contain genetic information? | a small circular plasmid and a large circular chromosome |
| What is the chromosome and the plasmid made from? | genes, each one coding for a different protein |
| What is genetic engineering? | scientists can now remove genes from the plasmid and also insert specific genes from other organisms into it. |
| Stage 1 of genetic engineering | identify the section of DNA that contains the required gene from the source chromosome |
| Stage 2 of genetic engineering | extract the required gene using a restriction endonuclease enzyme |
| Stage 3 of genetic engineering | insert the required gene into a bacterial plasmid using a ligase enzyme, the plasmid is the vector (carrier of genetic info) |
| Stage 4 of genetic engineering | insert the plasmid into a host cell (a bacterium) |
| Stage 5 of genetic engineering | culture the transformed cell to produce the required product/protein |