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Genetics

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Question:

Describe the process of replication

Author: H K



Answer:

Helicase unwinds helical shaped of DNA forming the origin of replication. Primase adds RNA primers on with 3'-OH ends necessary for the commencation of DNA polymerase to be able to synthesize DNA. DNA polymerase starts synthesizing DNA complement of both strands of parents, Because the two strands are antiparallel, continuous synthesis in the direction of the replication fork is possible along only one strand called the leading strand, whereas in the other strand the synthesis is discontinuous called lagging strand. RNA primers are removed by ligase, gaps temporarily created must be filled with DNA polymerase leaving some gaps. The gaps left after synthesis are filled and must be joined to the adjacent DNA strand by ligase.


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H K
H K