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BIOLOGY TOPIC 7 NUCLEIC ACIDS (HL)

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Question:

What is PCR? (polymerase chain reaction) (3 steps)

Author: Sammy Universe

What is PCR? (polymerase chain reaction) (3 steps)



Answer:

Artificial method of replication to make large quantities of a DNA sequence. happens in a thermal cycler at different temps 1) Denaturation: heat separates strands (95 degrees) 2) Annealing: cooled so primers make copying sequence (55 degrees) 3) Elongation: TAQ polymerase copies sequence (72 degrees) = 1 billion copies


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