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From course:

CLINCHEM LIPIDS LAB

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Question:

Principle of HDL Assay

Author: Ellyn Mae Armada



Answer:

The reaction between cholesterol other than HDL and the enzyme for cholesterol assay is suppressed by the electrostatic interaction between polyanions & cationic substances. • Hydrogen peroxide is formed by the free cholesterol in HDL by cholesterol oxidase. Oxidative condensation of EMSE and 4 AA is caused by hydrogen peroxide in the presence of peroxidase , and the absorbance of the resulting red purple quinone is measured to obtain the cholesterol value in HDL.


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