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From course:

A Level Biology Edexcel: Topic 5 - 8

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Question:

PCR process

Author: seremi amune



Answer:

Mix the DNA sample, primers, free nucleotides and DNA polymerase heat to 95°C to break the hydrogen bonds and separate the two strands cool to 50°C so that the primers can bind to the separated strands heat to 70°C so that DNA polymerase creates a copy of the sample by complementary base pairing using the free nucleotides leave the mix for about 1 min so the sample can be amplified the cycle can be repeated so that there is enough DNA for a DNA profile


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