Question:
Lap score principle
Author: Ellyn Mae ArmadaAnswer:
Alkaline phosphatase activity is present in varying degrees in the neutrophil and band form of the granulocytes The amount of dye precipitated is proportional to the amount of enzyme present. Generally, a high WBC with increased neutrophils is associated with increased enzyme. Relationship between the number of enzyme and the Neutrophil present is Directly proportional With the use of different substrate solution with the presence of Enzyme Alkaline Phosphatase in an alkaline ph, it will be hydrolyzed. Using working solution (ex. Fast Blue RR dye or Fast Blue BBN) it will result to an insoluble precipitate at the site of enzyme activity.
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