Question:
SBB Procedure
Author: Ellyn Mae ArmadaAnswer:
Fixair-dried films in cold buffered formalin-acetone fixative for 30 to 60 seconds Washin running water for 1 minute Immersein working stain for 1 hour Rinsein70% ethanol for 2 minutes Washin distilled water for 2 minutes Counterstainwith nuclear fast red 10 to 15 minutes Wash,fan dry and apply coverslip with glycerol mounting medium for examination
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Author
Ellyn Mae Armada