Splicing Mechanism

1. U1 snRNP forms p[airs with 5' splice junction 2. BBP binds with U2AF at the branch point 3. U2 snRNP displaces BBP and U2AF and binds a the the branching point 4. U4/U6/U5 enter 5.U4 and U6 bind tightly together by bps interactions 6.After RNA-RNA arrangements the bond breaks 7. U6 displaces U1 at 5' splice junction 8. Forms an active site for 2nd reaction