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MBS1002 Biomedical Approaches

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Question:

What is a tissue-based assay? and what is an example?

Author: Mirte Schattorie



Answer:

A tissue-based assay is an experiment that is done on a tissue and gives you insights in tissue characteristics. it is often done for detecting neuronal antibodies. 1. you have a tissue that you want to study. 2. you add a sample that contains autoantibodies. 3. if the antigen is present, the autoantibodies will bind. 4. you add a secondary antibody conjugated with an enzyme. 5. you add a substrate to visualise the reaction.


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