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Bio 102 Quiz #6 Review

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Bio 102 Quiz #6 Review

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Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. White colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ+ gene is ___

recombinant; disrupted by the inserted DNA fragment

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45 questions
When cloning DNA into bacteria, the ____
DNA sequence is inserted into a plasmid
The key elements of a PCR reaction are ____
DNA with the target sequence to be amplified, a pair of primers, the four nucleoside triphosphates, and DNA polymerase
Restriction fragment length polymorphisms (RFLPs) are used to ____
Compare the DNA sequences between individuals by looking for changes in restriction enzyme digest patterns
In which situation would gene cloning occur?
A DNA fragment is inserted into a plasmid, which is then introduced to a bacterium where the plasmid replicates.
What is the natural function of restriction endonucleases?
Defense against viruses that infect bacteria
Why are numerous replication cycles – each performed at three difference temperatures – a necessary part of a PCR reaction?
The initial cycles do not produce many copies of the target sequence, but since the number of molecules produced doubles with each cycle, millions of copies are produced after 20-30 cycles.
Plasmid cloning vectors generally contain genes for which two traits?
Β-galactosidase enzyme production and ampicillin resistance
The poly(A) tail of an mRNA ____
Protects the mRNA from attack by RNA-digesting enzymes
Plasmids are ____
Circular DNA molecules that replicate separately from chromosomal DNA
Amino-acid coding sequence retained in mature mRNA
Indicates the end of transcription in prokaryotes
Stop codon
Indicates the end of translation
Control sequence ahead of a transcription unit
Start codon
First codon read in translation
Any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose
Cartagena Protocol Biosafety
An international agreement on the safe use and handling of GMOs.
Short tandom repeat
Short 2–6 bp sequences repeated in series
Recombinant DNA
DNA from two different sources that have been joined together into a single molecule
Restriction Fragments
The products of restriction endonuclease action.
DNA Fingerprinting
Using STR loci, this technique revealed that the source of Dolly's genomic DNA was the ewe who supplied the mammary cell
Restriction Endonucleases
Bacterial enzymes that recognize and cut specific DNA sequences.
This procedure can be described as a photocopy machine for specific DNA sequences.
Genetic Engineering
DNA technology used to alter genes in a cell or organism.
Agarose gel electrophoresis
This technique is used to separate DNA molecules based on their relative sizes.
This term is used to describe organisms that have been modified to contain genetic information from an external source