How do we collect CSF?

Cerebrospinal fluid is collected aseptically from lumbar puncture. After proper antiseptic treatment of skin in lumbar area, the CSF sample is collected to sterile tubes preheated to 37°C. One part of CSF should be sent for direct microscopy. CSF should be sent immediately to the lab (in time no longer than 15 min in 37°C). In case of prolongation of transport (in 2h) temperature 37°C should be kept and never lowered under 30°C. For bacteriological culture, CSF should be also additionally added to a transport medium (preheated to 37°C) and sent in 37°C.