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MBS1002 Biomedical Approaches

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Question:

What are the cycles of PCR?

Author: Mirte Schattorie



Answer:

0. initial denaturation: uncoiling the helical structure of DNA. 1. denaturation: separation of dsDNA strands by heat (95 degrees). 2. annealing: attachment of primers at 50 to 65 degrees. 3. extension: synthesis of new DNA by DNA polymerase and nucleoside triphosphates at 72 degrees. 4. final extension


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